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Image Search Results


Journal: Cancer Cell

Article Title: An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer

doi: 10.1016/j.ccell.2019.08.008

Figure Lengend Snippet:

Article Snippet: To generate lentiviral EED and EZH2 expression plasmids, wild-type EED was PCR amplified from pCMV HA EED WT (Addgene 24231, a gift from Kristian Helin) ( ) and subcloned into pHRSIN-P SFFV -eGFP-P PGK -Puro via BamHI and XhoI, replacing the GFP.

Techniques: Transduction, Recombinant, Sequencing, CRISPR, Knock-Out, Software, Activity Assay